美國MEFs Oct4-GFP報告基因小鼠胚胎成纖維細胞

美國PrimCells干細胞：PrimCells提供多種報告基因敲進的小鼠胚胎干細胞，該類細胞皆在原始基因座上敲入熒光報告基因，從而使熒光蛋白的表達能真實反映原基因座附近的表達調(diào)控狀況。該類細胞作為研究胚胎干細胞自更新和定向分化的有力工具，受到多家有名研究所研究人員的青睞。

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Introduction:

Primary mouse embryonic fibroblasts (MEFs) are typically derived from E13.5 mouse embryos. They are relatively easy to culture and maintain and have robust proliferation rate. MEFs can be powerful tools to study gene functions especially when comparison experiments between wild-type and knockout cells from the same liter are required. Meanwhile, MEFs have also been used as feeder cells to support growth of embryonic stem cells from a variety of species1. More recently, MEFs from reporter transgenic mice were used for assessing gene function during reprogramming of fibroblasts to induced pluripotent stem cells (iPSCs) ².

PrimCells is proud to isolate and provide the primary cultures of embryonic fibroblasts from transgenic mice carrying IRES-GFP gene in their endogenous Pou5f1 (Oct4) locus. When these cells are fully reprogrammed, iPSCs will become GFP+, which could be a powerful tool for chemical screening or gene functional studies in order to develop more efficient reprogramming technology.

Figure 1 | Successfully reprogrammed iPSCs become GFP positive

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